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pcmv myc vector  (TaKaRa)


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    Structured Review

    TaKaRa pcmv myc vector
    Pcmv Myc Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 756 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+myc/pCMV-Myc+Vector+Set/pmc13179329-189-59-61
    Average 96 stars, based on 756 article reviews
    pcmv myc vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Generated:

    Article Title: SFPQ directs histone H3.3 deposition to R-loops in DNA repeats to protect genome stability.
    Article Snippet: SFPQ vectors purchased from Addgene are listed in Supplementary data 9. .. SFPQ P was generated by overlap extension PCR using primers containing KpnI and XhoI restriction sites for cloning the cDNA element into KpnI and XhoI sites of pCMV-Myc (ClonTech). pLV-eGFP was kindly provided by Prof. Giannino Del Sal (University of Trieste, Italy) and used for subsequent cloning. ..

    Article Title: The oncoprotein SET promotes serine-derived one-carbon metabolism by regulating SHMT2 enzymatic activity
    Article Snippet: .. Eukaryotic expression constructs were generated by cloning each cDNA into the pcDNA3.1 (Invitrogen K4800), pCMV-Myc (Clontech, 635689) or pWG (a modified pIRESneo2, Clontech, 6938-1) vector. .. The constructs expressing shRNA or sgRNA were generated by inserting the indicated oligos into the pLKO.1 (Addgene, 10878) or LentiCRISPRv2 (Addgene, 167932) vector.

    Polymerase Chain Reaction:

    Article Title: SFPQ directs histone H3.3 deposition to R-loops in DNA repeats to protect genome stability.
    Article Snippet: SFPQ vectors purchased from Addgene are listed in Supplementary data 9. .. SFPQ P was generated by overlap extension PCR using primers containing KpnI and XhoI restriction sites for cloning the cDNA element into KpnI and XhoI sites of pCMV-Myc (ClonTech). pLV-eGFP was kindly provided by Prof. Giannino Del Sal (University of Trieste, Italy) and used for subsequent cloning. ..

    Article Title: Male-Biased Cyp17a2 Governs Antiviral Sexual Dimorphism in Fish via STING Stabilization and Viral Protein Degradation
    Article Snippet: The sequences of zebrafish and gibel carp ( Carassius gibelio ) Cyp17a2 (GenBank accession number: NM_001105670.1 and XM_026199804.1) were obtained from the National Centre for Biotechnology Information (NCBI) website. .. Cyp17a2 was amplified by polymerase chain reaction (PCR) using cDNA from adult zebrafish or gibel carp tissues as a template and cloned into the expression vector pCMV-HA or pCMV-Myc (Clontech) vectors. ..

    Article Title: E3 ubiquitin ligase TRIM38 regulates macrophage polarization to reduce hepatic inflammation by interacting with HSPA5.
    Article Snippet: .. Full-length TRIM38 or HSPA5 was PCR-amplified from human or mouse cDNA and subcloned to pCMV-3× Flag (Sigma-Aldrich, #OGS627) or pCMV-Myc (Clontech, Mountain View, CA, USA, #635689). ..

    Cloning:

    Article Title: SFPQ directs histone H3.3 deposition to R-loops in DNA repeats to protect genome stability.
    Article Snippet: SFPQ vectors purchased from Addgene are listed in Supplementary data 9. .. SFPQ P was generated by overlap extension PCR using primers containing KpnI and XhoI restriction sites for cloning the cDNA element into KpnI and XhoI sites of pCMV-Myc (ClonTech). pLV-eGFP was kindly provided by Prof. Giannino Del Sal (University of Trieste, Italy) and used for subsequent cloning. ..

    Article Title: The oncoprotein SET promotes serine-derived one-carbon metabolism by regulating SHMT2 enzymatic activity
    Article Snippet: .. Eukaryotic expression constructs were generated by cloning each cDNA into the pcDNA3.1 (Invitrogen K4800), pCMV-Myc (Clontech, 635689) or pWG (a modified pIRESneo2, Clontech, 6938-1) vector. .. The constructs expressing shRNA or sgRNA were generated by inserting the indicated oligos into the pLKO.1 (Addgene, 10878) or LentiCRISPRv2 (Addgene, 167932) vector.

    Article Title: MOV10 inhibits SADS-CoV replication by enhancing TRIM24-mediated K63-linked TRAF3 ubiquitination and this inhibition is antagonized by viral N protein
    Article Snippet: .. The DNA fragment of MOV10 was inserted into the pCAGGS-HA (P0166; Miaoling Biology) or pCMV-Myc (635689; Clontech) vector via homologous recombination using the ClonExpress ultra one-step cloning kit (C116; Vazyme, Nanjing, China). ..

    Amplification:

    Article Title: Male-Biased Cyp17a2 Governs Antiviral Sexual Dimorphism in Fish via STING Stabilization and Viral Protein Degradation
    Article Snippet: The sequences of zebrafish and gibel carp ( Carassius gibelio ) Cyp17a2 (GenBank accession number: NM_001105670.1 and XM_026199804.1) were obtained from the National Centre for Biotechnology Information (NCBI) website. .. Cyp17a2 was amplified by polymerase chain reaction (PCR) using cDNA from adult zebrafish or gibel carp tissues as a template and cloned into the expression vector pCMV-HA or pCMV-Myc (Clontech) vectors. ..

    Article Title: The tumor suppressor CYLD acts as a deubiquitinase for mTOR to constrain its activity
    Article Snippet: The pcDNA3- myc-CYLD-C601S, pcDNA3-FLAG-mTOR-T631G, pcDNA3-FLAG-mTOR-3xKR (K777/782/784R) and pcDNA3-FLAG-mTOR-K2066R expression vector were generated by PCR amplification using appropriate oligos and the NEBuilder HiFi DNA Assembly Master Mix (#E2621, New England BioLabs). .. In brief, CYLD-C601S was amplified from the pcDNA3-FLAG-CYLD-C601S plasmid and assembled into the EcoRI/NotI sites of pCMV-myc (#635689, Clontech). ..

    Clone Assay:

    Article Title: Male-Biased Cyp17a2 Governs Antiviral Sexual Dimorphism in Fish via STING Stabilization and Viral Protein Degradation
    Article Snippet: The sequences of zebrafish and gibel carp ( Carassius gibelio ) Cyp17a2 (GenBank accession number: NM_001105670.1 and XM_026199804.1) were obtained from the National Centre for Biotechnology Information (NCBI) website. .. Cyp17a2 was amplified by polymerase chain reaction (PCR) using cDNA from adult zebrafish or gibel carp tissues as a template and cloned into the expression vector pCMV-HA or pCMV-Myc (Clontech) vectors. ..

    Expressing:

    Article Title: Male-Biased Cyp17a2 Governs Antiviral Sexual Dimorphism in Fish via STING Stabilization and Viral Protein Degradation
    Article Snippet: The sequences of zebrafish and gibel carp ( Carassius gibelio ) Cyp17a2 (GenBank accession number: NM_001105670.1 and XM_026199804.1) were obtained from the National Centre for Biotechnology Information (NCBI) website. .. Cyp17a2 was amplified by polymerase chain reaction (PCR) using cDNA from adult zebrafish or gibel carp tissues as a template and cloned into the expression vector pCMV-HA or pCMV-Myc (Clontech) vectors. ..

    Article Title: The oncoprotein SET promotes serine-derived one-carbon metabolism by regulating SHMT2 enzymatic activity
    Article Snippet: .. Eukaryotic expression constructs were generated by cloning each cDNA into the pcDNA3.1 (Invitrogen K4800), pCMV-Myc (Clontech, 635689) or pWG (a modified pIRESneo2, Clontech, 6938-1) vector. .. The constructs expressing shRNA or sgRNA were generated by inserting the indicated oligos into the pLKO.1 (Addgene, 10878) or LentiCRISPRv2 (Addgene, 167932) vector.

    Plasmid Preparation:

    Article Title: Male-Biased Cyp17a2 Governs Antiviral Sexual Dimorphism in Fish via STING Stabilization and Viral Protein Degradation
    Article Snippet: The sequences of zebrafish and gibel carp ( Carassius gibelio ) Cyp17a2 (GenBank accession number: NM_001105670.1 and XM_026199804.1) were obtained from the National Centre for Biotechnology Information (NCBI) website. .. Cyp17a2 was amplified by polymerase chain reaction (PCR) using cDNA from adult zebrafish or gibel carp tissues as a template and cloned into the expression vector pCMV-HA or pCMV-Myc (Clontech) vectors. ..

    Article Title: The tumor suppressor CYLD acts as a deubiquitinase for mTOR to constrain its activity
    Article Snippet: The pcDNA3- myc-CYLD-C601S, pcDNA3-FLAG-mTOR-T631G, pcDNA3-FLAG-mTOR-3xKR (K777/782/784R) and pcDNA3-FLAG-mTOR-K2066R expression vector were generated by PCR amplification using appropriate oligos and the NEBuilder HiFi DNA Assembly Master Mix (#E2621, New England BioLabs). .. In brief, CYLD-C601S was amplified from the pcDNA3-FLAG-CYLD-C601S plasmid and assembled into the EcoRI/NotI sites of pCMV-myc (#635689, Clontech). ..

    Construct:

    Article Title: The oncoprotein SET promotes serine-derived one-carbon metabolism by regulating SHMT2 enzymatic activity
    Article Snippet: .. Eukaryotic expression constructs were generated by cloning each cDNA into the pcDNA3.1 (Invitrogen K4800), pCMV-Myc (Clontech, 635689) or pWG (a modified pIRESneo2, Clontech, 6938-1) vector. .. The constructs expressing shRNA or sgRNA were generated by inserting the indicated oligos into the pLKO.1 (Addgene, 10878) or LentiCRISPRv2 (Addgene, 167932) vector.

    Modification:

    Article Title: The oncoprotein SET promotes serine-derived one-carbon metabolism by regulating SHMT2 enzymatic activity
    Article Snippet: .. Eukaryotic expression constructs were generated by cloning each cDNA into the pcDNA3.1 (Invitrogen K4800), pCMV-Myc (Clontech, 635689) or pWG (a modified pIRESneo2, Clontech, 6938-1) vector. .. The constructs expressing shRNA or sgRNA were generated by inserting the indicated oligos into the pLKO.1 (Addgene, 10878) or LentiCRISPRv2 (Addgene, 167932) vector.

    Homologous Recombination:

    Article Title: MOV10 inhibits SADS-CoV replication by enhancing TRIM24-mediated K63-linked TRAF3 ubiquitination and this inhibition is antagonized by viral N protein
    Article Snippet: .. The DNA fragment of MOV10 was inserted into the pCAGGS-HA (P0166; Miaoling Biology) or pCMV-Myc (635689; Clontech) vector via homologous recombination using the ClonExpress ultra one-step cloning kit (C116; Vazyme, Nanjing, China). ..

    other:

    Article Title: Arginine methylation-dependent METTL14-SMN interaction regulates RNA m 6 A homeostasis
    Article Snippet: pCMV-Myc , Takara , 635689.

    Western Blot:

    Article Title: Zinc fingers are responsible for the efficient control of KLF7 on the transcription of genes in the NF-κB signaling pathway and fatty acid β-oxidation.
    Article Snippet: .. Western blot analysis in the HEK293T, Ishikawa, HeLa, and EC109 cells (Pricella, Wuhan, China) showed that, unlike cells transfected with the empty vector (EV) of pCMV-myc (Clontech, Mountain View, USA), Myc-tagged proteins appeared at expected sizes in cells transfected with either the wild-type KLF7 or any of the three mutant KLF7 overexpression plasmids after 48 h (Supplementary Figure S1 and Figure 1A). ..

    Transfection:

    Article Title: Zinc fingers are responsible for the efficient control of KLF7 on the transcription of genes in the NF-κB signaling pathway and fatty acid β-oxidation.
    Article Snippet: .. Western blot analysis in the HEK293T, Ishikawa, HeLa, and EC109 cells (Pricella, Wuhan, China) showed that, unlike cells transfected with the empty vector (EV) of pCMV-myc (Clontech, Mountain View, USA), Myc-tagged proteins appeared at expected sizes in cells transfected with either the wild-type KLF7 or any of the three mutant KLF7 overexpression plasmids after 48 h (Supplementary Figure S1 and Figure 1A). ..

    Mutagenesis:

    Article Title: Zinc fingers are responsible for the efficient control of KLF7 on the transcription of genes in the NF-κB signaling pathway and fatty acid β-oxidation.
    Article Snippet: .. Western blot analysis in the HEK293T, Ishikawa, HeLa, and EC109 cells (Pricella, Wuhan, China) showed that, unlike cells transfected with the empty vector (EV) of pCMV-myc (Clontech, Mountain View, USA), Myc-tagged proteins appeared at expected sizes in cells transfected with either the wild-type KLF7 or any of the three mutant KLF7 overexpression plasmids after 48 h (Supplementary Figure S1 and Figure 1A). ..

    Over Expression:

    Article Title: Zinc fingers are responsible for the efficient control of KLF7 on the transcription of genes in the NF-κB signaling pathway and fatty acid β-oxidation.
    Article Snippet: .. Western blot analysis in the HEK293T, Ishikawa, HeLa, and EC109 cells (Pricella, Wuhan, China) showed that, unlike cells transfected with the empty vector (EV) of pCMV-myc (Clontech, Mountain View, USA), Myc-tagged proteins appeared at expected sizes in cells transfected with either the wild-type KLF7 or any of the three mutant KLF7 overexpression plasmids after 48 h (Supplementary Figure S1 and Figure 1A). ..



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